Evidence of Bacterial Metabolic Activity in Culture-Negative Otitis Media With Effusion
نویسندگان
چکیده
منابع مشابه
Evidence of bacterial metabolic activity in culture-negative otitis media with effusion.
CONTEXT Otitis media with effusion (OME) can lead to significant hearing loss in children. Although previous studies have shown that bacterial DNA is present in a significant percentage of effusions sterile by culture, whether the DNA represents viable organisms or "fossilized remains" is unknown. OBJECTIVE To determine if bacterial messenger RNA (mRNA), as detected by a reverse transcriptase...
متن کاملBacterial involvement in otitis media with effusion.
OBJECTIVE Otitis media with effusion (OME), a common chronic childhood condition affecting hearing, is thought to be a result of bacterial infection, with biofilms recently implicated. Although bacterial DNA can be detected by polymerase chain reaction in 80% of patients, typically fewer than half of effusions are positive using standard culture techniques. We adopted an alternative approach to...
متن کاملSource of bacterial RNA in chronic otitis media with effusion.
The purpose of this study was to investigate whether the bacterial RNA detected by polymerase chain reaction (PCR) and reverse transcription (RT)-PCR methods in middle ear effusion (MEE) for pediatric chronic otitis media with effusion (OME) originated from live bacteria. Degradation of RNA was observed by spectroscopic analysis; we also investigated the effect of MEE on the digestive activity ...
متن کاملOtitis Media with Effusion
Otitis media with effusion (OME), also called glue ear, is characterised by a collection of fluid in the middle-ear cleft. there is chronic inflammation but without signs of acute inflammation. OME is the most common cause of hearing impairment (and the most common reason for elective surgery) in childhood, where it usually follows an episode of acute otitis media (AOM). It is uncommon in adult...
متن کاملOtitis Media with Effusion
Otitis media with effusion (OME), also called glue ear, is characterised by a collection of fluid in the middle-ear cleft. there is chronic inflammation but without signs of acute inflammation. OME is the most common cause of hearing impairment (and the most common reason for elective surgery) in childhood, where it usually follows an episode of acute otitis media (AOM). It is uncommon in adult...
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ژورنال
عنوان ژورنال: JAMA
سال: 1998
ISSN: 0098-7484
DOI: 10.1001/jama.279.4.296